中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (29): 5441-5444.doi: 10.3969/j.issn. 2095-4344.2012.29. 025

• 药物控释材料 drug delivery materials • 上一篇    下一篇

冻干法制备的海藻酸钙-壳聚糖组织工程复合材料支架

李沁华，王 迪   

  1. 暨南大学生物医学工程系，广东省广州市 510632
  • 收稿日期:2011-12-17 修回日期:2012-02-06 出版日期:2012-07-15 发布日期:2012-07-15
  • 作者简介:李沁华★，女，1963年生，广东省广州市人，汉族，1987年暨南大学毕业，硕士，副研究员，主要从事组织工程生物材料支架与再生医学的研究。 libmejnu@ 163.com

Preparation of calcium alginate-chitosan tissue engineering scaffolds by a freeze-drying method

Li Qin-hua, Wang Di   

  1. Department of Biomedical Engineering, Jinan University, Guangzhou 510632, Guangdong Province, China
  • Received:2011-12-17 Revised:2012-02-06 Online:2012-07-15 Published:2012-07-15
  • About author:Li Qin-hua★, Master, Associate researcher, Department of Biomedical Engineering, Jinan University, Guangzhou 510632, Guangdong Province, China libmejnu@163.com

PDF

527

被引次数

8

摘要：

背景：高含水率与复合支架材料的组成有关，海藻酸钠是吸水性很高的高分子材料，而壳聚糖也具有亲水性。两者的复合保持了海藻酸钠高含水率的特性。
目的：以壳聚糖、海藻酸钠为主要成分，构建组织工程复合支架材料。
方法：采用正交试验法和单因子试验法，将壳聚糖和海藻酸钠按不同的配比复合，以不同的方法成型，测定复合支架的含水率、膨胀率；扫描电镜观察样品横截面的组织形态。
结果与结论：不同配比的壳聚糖与海藻酸钠复合，膜成型法得到的材料含水率77.20%，冷冻干燥法得到的支架材料含水率最高75.27%，而微球颗粒的含水率高达97.13%。说明不同配比的复合支架材料具有不同的内部孔洞结构，不同成型法对材料性能影响不同。海藻酸钙-壳聚糖复合支架材料具有较高的含水率，内部孔洞结构丰富，可作为组织工程支架材料。

关键词: 海藻酸钠, 壳聚糖, 组织工程, 复合支架, 膜成型法, 生物材料

Abstract:

BACKGROUND: High water content is related to the composition of composite scaffolds. Sodium alginate is a polymer material with high water absorption, and chitosan has hydrophilicity. Therefore, the combination of these two materials has high water content as sodium alginate.
OBJECTIVE: To construct calcium alginate-chitosan composite scaffolds for tissue engineering by mainly using chitosan and sodium alginate.
METHODS: Chitosan and sodium alginate were compounded in various proportions and formed in different ways by orthogonal experiment and single factor experiment methods. The water content and swelling ratio of the composite scaffolds were measured. The histological morphology of the cross-sections of scaffolds was observed by scanning electron microscope.
RESULTS AND CONCLUSION: The water content of the scaffolds was 77.20% for the film-forming method, 75.27% for the freeze-drying method, and 97.13% for particle forming method. These findings suggest that the scaffolds prepared in various ways have different cellular structures. Different forming methods have different influences on scaffold properties. Calcium alginate-chitosan scaffolds have high water content and swelling ratio as well as abundant cellular structures, which can be used as tissue engineering scaffolds.

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